RegulonDB RegulonDB 11.2: Gene Form
   

mdtF gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

mdtF mdtE gadW

Gene      
Name: mdtF    Texpresso search in the literature
Synonym(s): ECK3498, EG12241, b3514, yhiV
Genome position(nucleotides): 3660414 --> 3663527
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 53.02
External database links:  
ASAP:
 ABE-0011480
ECHOBASE:
 EB2152
ECOLIHUB:
 mdtF
OU-MICROARRAY:
 b3514
STRING:
 511145.b3514
COLOMBOS: mdtF


Product      
Name: multidrug efflux pump RND permease MdtF
Synonym(s): MdtF, YhiV
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane
Molecular weight: 111.517
Isoelectric point: 5.105
Motif(s):
 
Type Positions Sequence Comment
1 -> 1027 MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTITVSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDIAQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVASNIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQLGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYSTVARYNGKPAAGIAIKLAAGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIEISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINTLTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLSAVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPNALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEEDQGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGLAFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMELLDNGNLGHEKLTQARNELLSLAAQSPNQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVALSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAPLSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYSWTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLATDLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRPILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVV
10 -> 28 VFAWVLAIIMMLAGGLAIM UniProt: Helical; Name=1.
340 -> 359 VFKTLVEAIILVFLVMYLFL UniProt: Helical; Name=2.
366 -> 385 IIPTIAVPVVILGTFAILSA UniProt: Helical; Name=3.
392 -> 413 TLTMFGMVLAIGLLVDDAIVVV UniProt: Helical; Name=4.

 
Classification:
Multifun Terms (GenProtEC)  
  4 - transport --> 4.2 - Electrochemical potential driven transporters
  5 - cell processes --> 5.6 - protection --> 5.6.4 - drug resistance/sensitivity
  6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0005887 - integral component of plasma membrane
GO:0016021 - integral component of membrane
molecular_function GO:0015125 - bile acid transmembrane transporter activity
GO:0015562 - efflux transmembrane transporter activity
GO:0022857 - transmembrane transporter activity
GO:0042802 - identical protein binding
GO:0042910 - xenobiotic transmembrane transporter activity
biological_process GO:0009636 - response to toxic substance
GO:0046677 - response to antibiotic
GO:0006855 - xenobiotic transmembrane transport
GO:0055085 - transmembrane transport
GO:0042908 - xenobiotic transport
GO:0015721 - bile acid and bile salt transport
Note(s): Note(s): ...[more].
Reference(s): [1] Alon Cudkowicz N., et al., 2019
[2] Bohnert JA., et al., 2007
[3] Shuster Y., et al., 2016
External database links:  
ALPHAFOLD:
 P37637
ECOCYC:
 YHIV-MONOMER
ECOLIWIKI:
 b3514
INTERPRO:
 IPR004764
INTERPRO:
 IPR027463
INTERPRO:
 IPR001036
MODBASE:
 P37637
PANTHER:
 PTHR32063
PFAM:
 PF00873
PRIDE:
 P37637
PRINTS:
 PR00702
PRODB:
 PRO_000023186
REFSEQ:
 NP_417971
SMR:
 P37637
SWISSMODEL:
 P37637
UNIPROT:
 P37637


Operon      
Name: gadEF-mdtEF         
Operon arrangement:
Transcription unit        Promoter
gadEF
gadE-mdtEF
gadEF
gadEF-mdtEF
mdtEF
mdtEF
mdtEF


Transcriptional Regulation      
Display Regulation             
Activated by: EvgA, GadX, YdeO, GadW, GadE
Repressed by: CRP, FliZ, H-NS


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References


Reference(s)    

 [1] Alon Cudkowicz N., Schuldiner S., 2019, Deletion of the major Escherichia coli multidrug transporter AcrB reveals transporter plasticity and redundancy in bacterial cells., PLoS One 14(6):e0218828

 [2] Bohnert JA., Schuster S., Fahnrich E., Trittler R., Kern WV., 2007, Altered spectrum of multidrug resistance associated with a single point mutation in the Escherichia coli RND-type MDR efflux pump YhiV (MdtF)., J Antimicrob Chemother 59(6):1216-22

 [3] Shuster Y., Steiner-Mordoch S., Alon Cudkowicz N., Schuldiner S., 2016, A Transporter Interactome Is Essential for the Acquisition of Antimicrobial Resistance to Antibiotics., PLoS One 11(4):e0152917

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