RegulonDB RegulonDB 11.2: Operon Form
   

uhpT operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: uhpT
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: uhpT
Synonym(s): OP00173
Gene(s): uhpT   Genome Browser M3D Gene expression COLOMBOS
Note(s): uhpT gene expression is induced by glucose-6P or pyruvate and is inhibited by high phosphate concentrations Verhamme DT,2002
It has been observed that the transcriptional regulator IHF binds near the start gene of uhpT Kasho K,2021.
Reference(s): [1] Merkel TJ., et al., 1992
Promoter
Name: uhpTp
+1: 3847198
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 31
Sequence: ccaggttttgcctggacgctatctcaggcctgatttgctgctgatttttacaatgcatgcCtcacgcaggtattcatttca
                            -35                       -10   +1                   
Evidence: [COMP-AINF]
[COMP-HINF]
[COMP-HINF-POSITIONAL-IDENTIFICATION]
[EXP-IDA-TRANSCRIPTION-INIT-MAPPING]
Reference(s): [2] Huerta AM., et al., 2003
[1] Merkel TJ., et al., 1992
TF binding sites (TFBSs)
[CRP,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence Confidence level (C: Confirmed, S: Strong, W: Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote CRP-cyclic-AMP activator uhpTp 3847291 3847312 -103.5 acaccgcgcgAAGCGTGATGCATCTCACCTTTtcacttcata nd [EXP-IEP-GENE-EXPRESSION-ANALYSIS], [COMP-HINF-SIMILAR-TO-CONSENSUS], [EXP-IDA-BINDING-OF-CELLULAR-EXTRACTS], [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS], [EXP-IMP-SITE-MUTATION] C [3]
[UhpA,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence Confidence level (C: Confirmed, S: Strong, W: Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal UhpA-phosphorylated activator uhpTp 3847230 3847249 -41.5 tccaggttttGCCTGGACGCTATCTCAGGCctgatttgct nd [EXP-IEP-GENE-EXPRESSION-ANALYSIS], [EXP-IDA-BINDING-OF-CELLULAR-EXTRACTS], [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS], [EXP-IMP-SITE-MUTATION] C [3], [5], [6], [8]
proximal UhpA-phosphorylated activator uhpTp 3847253 3847272 -64.5 tatccggcaaAACTAAGAAATTTTCCAGGTtttgcctgga nd [EXP-IEP-GENE-EXPRESSION-ANALYSIS], [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS], [EXP-IMP-SITE-MUTATION] C [4], [5], [6], [7]
[UvrY,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence Confidence level (C: Confirmed, S: Strong, W: Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
nd UvrY-phosphorylated activator uhpTp nd nd nd nd nd [EXP-IEP-GENE-EXPRESSION-ANALYSIS], [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS] W [9]


Evidence    

 [COMP-AINF] Inferred computationally without human oversight

 [COMP-HINF] Inferred by a human based on computational evidence

 [COMP-HINF-POSITIONAL-IDENTIFICATION] Human inference of promoter position

 [EXP-IDA-TRANSCRIPTION-INIT-MAPPING] Transcription initiation mapping

 [EXP-IEP-GENE-EXPRESSION-ANALYSIS] Gene expression analysis

 [COMP-HINF-SIMILAR-TO-CONSENSUS] A person inferred or reviewed a computer inference of sequence function based on similarity to a consensus sequence.

 [EXP-IDA-BINDING-OF-CELLULAR-EXTRACTS] Binding of cellular extracts

 [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS] Binding of purified proteins

 [EXP-IMP-SITE-MUTATION] Site mutation


Reference(s)    

 [1] Merkel TJ., Nelson DM., Brauer CL., Kadner RJ., 1992, Promoter elements required for positive control of transcription of the Escherichia coli uhpT gene., J Bacteriol 174(9):2763-70

 [2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [3] Olekhnovich IN., Kadner RJ., 2002, Mutational scanning and affinity cleavage analysis of UhpA-binding sites in the Escherichia coli uhpT promoter., J Bacteriol 184(10):2682-91

 [4] Chen Q., Kadner RJ., 2000, Effect of altered spacing between uhpT promoter elements on transcription activation., J Bacteriol 182(16):4430-6

 [5] Dahl JL., Wei BY., Kadner RJ., 1997, Protein phosphorylation affects binding of the Escherichia coli transcription activator UhpA to the uhpT promoter., J Biol Chem 272(3):1910-9

 [6] Verhamme DT., Postma PW., Crielaard W., Hellingwerf KJ., 2002, Cooperativity in signal transfer through the Uhp system of Escherichia coli., J Bacteriol 184(15):4205-10

 [7] Wright JS., Olekhnovich IN., Touchie G., Kadner RJ., 2000, The histidine kinase domain of UhpB inhibits UhpA action at the Escherichia coli uhpT promoter., J Bacteriol 182(22):6279-86

 [8] Olekhnovich IN., Dahl JL., Kadner RJ., 1999, Separate contributions of UhpA and CAP to activation of transcription of the uhpT promoter of Escherichia coli., J Mol Biol 292(5):973-86

 [9] Yoshida M., Ishihama A., Yamamoto K., 2015, Cross talk in promoter recognition between six NarL-family response regulators of Escherichia coli two-component system., Genes Cells 20(7):601-12

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