RegulonDB RegulonDB 11.2: Operon Form
   

zntA operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: zntA
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: zntA
Gene(s): zntA   Genome Browser M3D Gene expression COLOMBOS
Note(s): Zinc induced activation of zntA transcription in a microarray analysis Yamamoto K,2005
Promoter
Name: zntAp
+1: 3606422
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 29
Sequence: ctcgctgtatctctgataaaacttgactctggagtcgactccagagtgtatccttcggttAatgagaaaaaacttaaccgg
                       -35                       -10        +1                   
Evidence: [COMP-AINF]
[COMP-HINF-POSITIONAL-IDENTIFICATION]
[EXP-IDA-TRANSCRIPTION-INIT-MAPPING]
[RS-EPT-CBR]
Reference(s): [1] Brocklehurst KR., et al., 1999
[2] Huerta AM., et al., 2003
[3] Outten CE., et al., 1999
[4] Salgado H, et al., 2012
TF binding sites (TFBSs)
[ZntR,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence Confidence level (C: Confirmed, S: Strong, W: Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal ZntR-Zn2+ activator zntAp 3606387 3606408 -24.5 ataaaacttgACTCTGGAGTCGACTCCAGAGTgtatccttcg nd [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS], [EXP-IMP-SITE-MUTATION] C [1], [3]


RNA cis-regulatory element    
Regulation, transcriptional elongation  
Attenuator type: Translational
Strand: forward
  Structure type Energy LeftPos RightPos Sequence (RNA-strand)
  terminator -19.9 3606409 3606449 ctccagagtgTATCCTTCGGTTAATGAGAAAAAACTTAACCGGAGGATGCcatgtcgact
Notes: "The provided "Sequence" is that of the RNA strand, i.e. U's are shown instead of T's and regulators on the reverse strand will appear as the reverse complement of the sequence delimited by LeftPos-RigtPos"


Evidence    

 [COMP-AINF] Inferred computationally without human oversight

 [COMP-HINF-POSITIONAL-IDENTIFICATION] Human inference of promoter position

 [EXP-IDA-TRANSCRIPTION-INIT-MAPPING] Transcription initiation mapping

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

 [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS] Binding of purified proteins

 [EXP-IMP-SITE-MUTATION] Site mutation


Reference(s)    

 [1] Brocklehurst KR., Hobman JL., Lawley B., Blank L., Marshall SJ., Brown NL., Morby AP., 1999, ZntR is a Zn(II)-responsive MerR-like transcriptional regulator of zntA in Escherichia coli., Mol Microbiol 31(3):893-902

 [2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [3] Outten CE., Outten FW., O'Halloran TV., 1999, DNA distortion mechanism for transcriptional activation by ZntR, a Zn(II)-responsive MerR homologue in Escherichia coli., J Biol Chem 274(53):37517-24

 [4] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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