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FrlR DNA-binding transcriptional repressor

Synonyms: FrlR, FrlR-N6-(1-deoxy-6-O-phospho-D-fructos-1-yl)-L-lysine
Summary:
As a dimer, FrlR binds DNA to regulate transcription, and it becomes inactive as a regulator when it is bound to fructoselysine-6-phosphate [1]. It has a winged helix-turn-helix domain for binding DNA, and this domain contains the amino acids Arg49, Gly70, and Lys71, which appear to be essential for DNA binding [1]. FrlR has similarity to Bacillus subtilis FrlR [1, 2], B. subtilis NagR [1], and S. enterica FraR [1]. As homologues, this protein appears to pertain to the GntR/HutC family of transcriptional regulators [1]. FrlR: fructolysine [2] Read more >


Transcription factor      
TF conformation(s):
Name Conformation Type TF-Effector Interaction Type Apo/Holo Conformation Evidence Confidence level (C: Confirmed, S: Strong, W: Weak) References
FrlR Functional   nd nd nd
FrlR-N6-(1-deoxy-6-O-phospho-D-fructos-1-yl)-L-lysine Non-Functional   nd nd nd
TFBs length: 10
Connectivity class: Local Regulator
Gene name: frlR
  Genome position: 3504052-3504783
  Length: 732 bp / 243 aa
Operon name: frlABCDR
TU(s) encoding the TF:
Transcription unit        Promoter
frlABCDR
frlAp1
frlR
frlRp1
frlR
frlRp2


Regulon       
Regulated gene(s) frlA, frlB, frlC, frlD, frlR
Multifun term(s) of regulated gene(s) MultiFun Term (List of genes associated to the multifun term)
carbon compounds (3)
Porters (Uni-, Sym- and Antiporters) (1)
membrane (1)
Transcription related (1)
repressor (1)
Regulated operon(s) frlABCDR
First gene in the operon(s) frlA
Simple and complex regulons CRP,FrlR
Simple and complex regulatory phrases Regulatory phrase (List of promoters regulated by the phrase)
[FrlR,-](1)


Transcription factor regulation    


Transcription factor binding sites (TFBSs) arrangements
      

  Functional conformation Function Promoter Sigma factor Central Rel-Pos Distance to first Gene Genes Sequence LeftPos RightPos Evidence Confidence level (C: Confirmed, S: Strong, W: Weak) References
  FrlR repressor frlAp1 Sigma70 12.5 -63.5 frlA, frlB, frlC, frlD, frlR
atcatgcattGTCATGTTACcttttaaatg
 3499842 3499851 [EXP-IEP-GENE-EXPRESSION-ANALYSIS], [COMP-HINF-SIMILAR-TO-CONSENSUS], [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS], [EXP-IMP-SITE-MUTATION] C [1], [1]


Evolutionary conservation of regulatory elements    
     Note: Evolutionary conservation of regulatory interactions and promoters is limited to gammaproteobacteria.
Promoter-target gene evolutionary conservation


Evidence    

 [EXP-IEP-GENE-EXPRESSION-ANALYSIS] Gene expression analysis

 [COMP-HINF-SIMILAR-TO-CONSENSUS] A person inferred or reviewed a computer inference of sequence function based on similarity to a consensus sequence.

 [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS] Binding of purified proteins

 [EXP-IMP-SITE-MUTATION] Site mutation


Reference(s)    

 [1] Graf von Armansperg B., Koller F., Gericke N., Hellwig M., Jagtap PKA., Heermann R., Hennig J., Henle T., Lassak J., 2021, Transcriptional regulation of the Nε -fructoselysine metabolism in Escherichia coli by global and substrate-specific cues., Mol Microbiol 115(2):175-190

 [2] Wiame E., Delpierre G., Collard F., Van Schaftingen E., 2002, Identification of a pathway for the utilization of the Amadori product fructoselysine in Escherichia coli., J Biol Chem 277(45):42523-9


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