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| Synonyms: MhpR-3-(2,3-dihydroxyphenyl)propionate [DHPP], MhpR, MhpR-3-(3-hydroxyphenyl)propanoate |
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Summary:
MhpR, "hydroxyphenylpropionate regulator," participates in controlling the expression of several genes involved in degradation of phenylpropionate (PP) [1, 2] MhpR is able to bind to its DNA-binding site even in the absence of inducer; however, increasing concentrations of inducer improve the binding strength of MhpR. 3-(2,3-Dihydroxyphenyl)propionate (DHPP) and 3-hydroxyphenylpropionate (3HPP), two intermediates of the metabolic pathways, are the principal inducers of MhpR. The third inducer, phenylpropionate (PP), acts synergistically with DHPP and 3HPP [1, 3] This protein, which belongs to the IclR family of transcriptional regulators, has a helix-turn-helix motif to bind DNA in its N-terminal domain and a 22-residue motif, specific to an IclR subfamily, in its C-terminal domain [2] MhpR is necessary to recruit CRP to the regulatory region of the mhp operon, where both proteins activate transcription [1] The mhpR gene, which is located downstream of the genes it regulates and in the opposite direction, appears to be expressed constitutively [1] Read more > |
| Transcription factor |  |
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| TF conformation(s): |
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| Evolutionary Family: | IclR | ||||||||||||||||||||||||||||
| TFBs length: | 15 | ||||||||||||||||||||||||||||
| Sensing class: | Sensing external and internal signals | ||||||||||||||||||||||||||||
| Connectivity class: | Local Regulator | ||||||||||||||||||||||||||||
| Gene name: | mhpR | ||||||||||||||||||||||||||||
| Genome position: | 367587-368420 | ||||||||||||||||||||||||||||
| Length: | 834 bp / 277 aa | ||||||||||||||||||||||||||||
| Operon name: | mhpR-lacI | ||||||||||||||||||||||||||||
| TU(s) encoding the TF: |
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| Regulon |
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| Regulated gene(s) | mhpA, mhpB, mhpC, mhpD, mhpE, mhpF | ||||||
| Multifun term(s) of regulated gene(s) |
MultiFun Term (List of genes associated to the multifun term)
carbon compounds (5)
fatty acids (1)
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| Regulated operon(s) | mhpABCDFE | ||||||
| First gene in the operon(s) | mhpA | ||||||
| Simple and complex regulons | CRP,MhpR | ||||||
| Simple and complex regulatory phrases | Regulatory phrase (List of promoters regulated by the phrase) | ||||||
| Transcription factor regulation |
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Transcription factor binding sites (TFBSs) arrangements
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| Functional conformation | Function | Promoter | Sigma factor | Central Rel-Pos | Distance to first Gene | Genes | Sequence | LeftPos | RightPos | Evidence | Confidence level (C: Confirmed, S: Strong, W: Weak) | References | |
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| MhpR-3-(3-hydroxyphenyl)propanoate | activator | mhpAp | Sigma70 | -58.0 | -150.0 | mhpA, mhpB, mhpC, mhpD, mhpF, mhpE |
aaacaacgtgGTGCACCTGGTGCACattcgggcat
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368454 | 368468 | [EXP-IEP-GENE-EXPRESSION-ANALYSIS], [COMP-HINF-SIMILAR-TO-CONSENSUS], [EXP-IDA-BINDING-OF-CELLULAR-EXTRACTS], [EXP-IMP-SITE-MUTATION] | C | [1] |
| Evolutionary conservation of regulatory elements | |
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| Promoter-target gene evolutionary conservation |
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| Reference(s) |
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[EXP-IEP-GENE-EXPRESSION-ANALYSIS] Gene expression analysis