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| Synonyms: ZntR-Zn2+, ZntR, ZntR-Cd2+, ZntR-Hg |
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Summary:
ZntR, "zinc transport regulator," regulates a gene involved in the Zn(II), Cd(II), and Pb(II) transport systems [1] In the presence of any of these ions, ZntR activates transcription of zntA by a DNA distortion mechanism that makes the promoter a better substrate for RNA polymerase [1, 2] The interaction of ZntR with Zn(II) has been characterized [2] but its interaction with other ions has not been described. An inverted repeat sequence of 22 bp, located in the spacer between the -10 and the -35 boxes, has been identified as the ZntR dimer-binding site [1, 2] The crystal structure of an N-terminally truncated fragment of ZntR bound to zinc was solved at a resolution of 19 Å [3] Each monomer of ZntR has three domains: a dimerization helix, a signal recognition site and a DNA-binding motif (helix-turn-helix) [1, 3] Two zinc ions are bound to each monomer [3] ZntR belongs to the MerR family, and although most members of this family are transcribed divergently from the genes they regulate, zntR is distant on the genome from its regulated gene [1] The ClpXP and Lon proteases degrade ZntR, but the regulator is protected against proteolysis when it is bound to zinc and DNA. Read more > |
| Transcription factor |  |
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| TF conformation(s): |
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| TFBs length: | 22 | |||||||||||||||||||||||||||||||||||
| TFBs symmetry: | inverted-repeat | |||||||||||||||||||||||||||||||||||
| Sensing class: | External sensing using transported metabolites | |||||||||||||||||||||||||||||||||||
| Connectivity class: | Local Regulator | |||||||||||||||||||||||||||||||||||
| Gene name: | zntR | |||||||||||||||||||||||||||||||||||
| Genome position: | 3438705-3439130 | |||||||||||||||||||||||||||||||||||
| Length: | 426 bp / 141 aa | |||||||||||||||||||||||||||||||||||
| Operon name: | yhdN-zntR | |||||||||||||||||||||||||||||||||||
| TU(s) encoding the TF: |
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| Regulon |
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| Regulated gene(s) | zntA | ||||||
| Multifun term(s) of regulated gene(s) |
MultiFun Term (List of genes associated to the multifun term)
ATP proton motive force interconversion (1)
The P-type ATPase (P-ATPase) Superfamily (1)
detoxification (1)
membrane (1)
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| Regulated operon(s) | zntA | ||||||
| First gene in the operon(s) | zntA | ||||||
| Simple and complex regulons | ZntR | ||||||
| Simple and complex regulatory phrases | Regulatory phrase (List of promoters regulated by the phrase) | ||||||
| Transcription factor regulation |
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Transcription factor binding sites (TFBSs) arrangements
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| Functional conformation | Function | Promoter | Sigma factor | Central Rel-Pos | Distance to first Gene | Genes | Sequence | LeftPos | RightPos | Evidence | Confidence level (C: Confirmed, S: Strong, W: Weak) | References | |
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| ZntR-Zn2+ | activator | zntAp | Sigma70 | -24.5 | -53.5 | zntA |
ataaaacttgACTCTGGAGTCGACTCCAGAGTgtatccttcg
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3606387 | 3606408 | [EXP-IDA-BINDING-OF-PURIFIED-PROTEINS], [EXP-IMP-SITE-MUTATION] | C | [1], [1], [2], [2] |
| Evolutionary conservation of regulatory elements | |
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| Promoter-target gene evolutionary conservation |
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| Reference(s) |
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[EXP-IDA-BINDING-OF-PURIFIED-PROTEINS] Binding of purified proteins